Spectroscopic and Time Domain Reflectometry Studies of Protein in 1, 2-Dihydroxyethane Solution
R. B. Talware *
K. J. Somaiya College of Arts, Commerce & Science, Kopargaon – 423601, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
A dielectric measurement was performed on proteins in an aqueous media using dielectric time domain spectroscopy above a comprehensive of frequency 10 MHz to 30 GHz. The static dielectric constant (ε0), relaxation time (t) and conductivity (\(\sigma\)) have been determined using nonlinear least squares fit technique. On the antagonistic 1, 2-dihydroxyethane (C2H6O2) intensely marks the dielectric reaction of lysozyme signifying a precise result on its dynamics. The data are consistently inferred assuming that glycol molecule blocks and separates the two domains of lysozyme making them rotationally self-determining. This paper explored the dielectric assets, as a purpose of incidence and temperature of the hydrated circular protein lysozyme.
Keywords: Dielectric constant, relaxation time, conductivity, time domain reflectometry